Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 20 pH (100 g/l) 11

20 pH (100 g/l) 11

0 Beef Extract 5

respectively 10% and 5% of the NaCl concentration is present in both formulations

Malt Extract 30 Adenine 0

MES was developed as one of Good's buffers in the 1960s

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 20 pH (100 g/l) 11Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room